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3 Calculation Modes

Peptide Reconstitution Calculator

Enter the peptide amount in the vial and the bacteriostatic water you add, and get the concentration and exact U-100 syringe units for any volume you need to draw.

Find Units

From amount → syringe units

Syringe size

Concentration

5,000mcg / mL

5.00 mg / mL

Draw volume0.100 mL
Syringe units (U-100)10.0 units
Draws per vial20

The formula

The math

Concentration = Vial (mg) ÷ BAC water (mL)

For example, a 10 mg vial mixed with 2 mL of BAC water yields 5,000 mcg/mL.

Units to draw = (target amount ÷ concentration) × 100

Drawing 500 mcg from a 5,000 mcg/mL solution takes 0.1 mL, which is 10 units on a U-100 syringe. The 1 mL, 0.5 mL and 0.3 mL sizes all use the same 100-units-per-mL scale; the smaller barrels just hold fewer units, which makes small volumes easier to read.

Find Amount and Find BAC Water rearrange the same equation: amount = (units ÷ 100) × concentration, and BAC water = vial (mcg) × units ÷ (amount × 100).

For research use only. This tool converts units; it does not recommend amounts. Check the vial size against the batch certificate of analysis for the product in hand.

Quick reference

Peptide Reconstitution Chart

Concentration for common vial sizes and water volumes, with the amount held in each unit on a U-100 syringe.

Concentration and amount per unit on a U-100 syringe, by vial size and water added
Vial sizeWater addedConcentrationPer unit (U-100)
5 mg1 mL5 mg/mL50 mcg
5 mg2 mL2.5 mg/mL25 mcg
5 mg3 mL1.67 mg/mL16.7 mcg
10 mg1 mL10 mg/mL100 mcg
10 mg2 mL5 mg/mL50 mcg
10 mg3 mL3.33 mg/mL33.3 mcg
20 mg1 mL20 mg/mL200 mcg
20 mg2 mL10 mg/mL100 mcg
20 mg3 mL6.67 mg/mL66.7 mcg

By compound

Reconstitution Calculator by Compound

The math is the same for every lyophilised peptide. Pick a compound to see its common vial sizes and current supplier prices.

Next step

Before You Buy, Compare

Know your concentration, now check the price. Compare cost per mg across suppliers with the price per mg calculator, see live listings in the price checker, and check batch lab reports before you choose.

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Questions

Frequently asked questions.

How does a peptide reconstitution calculator work?
It divides the peptide in the vial by the water you add to get concentration, then converts the volume you want to draw into syringe units. On a U-100 syringe, 100 units equals 1 ml, so each unit is 0.01 ml.
How much bacteriostatic water should I add to a peptide vial?
There is no single correct amount. More water gives a lower concentration, which makes small amounts easier to measure. Less water gives a stronger solution. For example, 10 mg in 2 ml is 5 mg/ml, so each unit on a U-100 syringe holds 0.05 mg (50 mcg).
How do I convert mg to mcg and syringe units?
1 mg equals 1,000 mcg. To get units, divide the amount you need by the concentration in mg/ml, then multiply by 100. The calculator does this automatically once you enter the vial size and water volume.
Does the calculator work for tirzepatide, retatrutide and other peptides?
Yes. The math is the same for any lyophilised peptide. Enter the milligrams printed on the vial label and the water you add, and the result applies whatever the compound.
Is this calculator dosing advice?
No. It only does the arithmetic of concentration and volume. It does not recommend amounts or protocols. All peptides listed on PepLookup are for research use only.